SeedGenes Profile: EMB 2407

Gene Information Gene Symbol EMB 2407 Chromosome Locus At5g60540   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol PDX 2 (T. Fitzpatrick) Sequence Identity Gene Class Embryo Defective Predicted Function Imidazoleglycerol-Phosphate Synthase Subunit H; Pyroxidine Amidotransferase Function Details Pyroxidine (vitamin B6) biosynthesis Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Fitzpatrick / Salk (1 allele) Border Recovery Fitzpatrick / Riken (1 allele) Database Release September, 2003 Predicted Sequences Gene: 2180 nucleotides mRNA: 1174 nucleotides Protein:   255 amino acids cDNA Status FLcDNA Available: AK117313 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   Neurospora crassa (44%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 247641_at

Mutant Information Allele Symbol emb 2407 pdx 2-1 pdx 2-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Fitzpatrick / Salk Fitzpatrick / Riken Mutant Line Number 85146 SALK_072168 RATM53-2381-1 Ecotype Columbia Columbia Nossen Genetic Background       ABRC Stock CS16214     Mutagen Treatment T-DNA T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Exon 2 Intron 1 Intron 1 Terminal Phenotype Cotyledon  Details Globular Globular Nomarski Phenotype   Images Allelism Evidence Reference Allele Sequence Identity Sequence Identity Database Release September, 2003 October, 2007 October, 2007 Seed Color White to Pale Yellow     Embryo Color White to Pale Yellow     Percent Mutant Seeds 26.2 %     Chi - Square 0.8     Seeds Counted 1086     Percent Top Half 51.1 %     Chi - Square 0.1     Average Seed Length 490 ± 70 µm     Average Embryo Length 160 ± 90 µm