SeedGenes Profile: EMB 2730

Gene Information Gene Symbol EMB 2730 Chromosome Locus At5g02250   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol RNR 1 (D. Stern) Sequence Identity RNR 1 (D. Stern) Complemetation Test Gene Class Embryo Defective Predicted Function Ribonuclease II Family Protein Function Details 3' to 5' exoribonuclease; Maturation of chloroplast rRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Border Recovery Stern / Salk (2 alleles) Border Recovery Meinke; Stern / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4745 nucleotides mRNA: 2911 nucleotides Protein:   803 amino acids cDNA Status FLcDNA Available: AK176383 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   Synechocystis (37%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251040_at

Mutant Information Allele Symbol emb 2730-1 emb 2730-2 Mutant Class Embryo Defective Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 11129 SALK_039364 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16126   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 14 to 3' UTR Exon 7 Terminal Phenotype Cotyledon  Details Pigment Defective  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 October, 2007 Seed Color White to Pale Yellow Pale Yellow Embryo Color White to Pale Yellow Pale Yellow Percent Mutant Seeds 24.0 % 23.7 % Chi - Square 0.6 0.3 Seeds Counted 1079 295 Percent Top Half 53.6 % 54.3 % Chi - Square 1.4 0.5 Average Seed Length 500 ± 40 µm 550 ± 0 µm Average Embryo Length 380 ± 60 µm 450 ± 0 µm