SeedGenes Profile: EMB 2759

Gene Information Gene Symbol EMB 2759 Chromosome Locus At5g63050   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details None Gene Evidence Double Border Recovery Meinke / Syngenta (3 alleles) Single Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2004 Predicted Sequences Gene: 2561 nucleotides mRNA: 1496 nucleotides Protein:   345 amino acids cDNA Status FLcDNA Available: BT011233 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (78%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 247414_at

Mutant Information Allele Symbol emb 2759-1 emb 2759-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 11833 16089 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 3 Intron 3 to Exon 4 Terminal Phenotype Transition  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2004 September, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 25.6 % 24.8 % Chi - Square 0.2 0.2 Seeds Counted 793 888 Percent Top Half 48.8 % 52.3 % Chi - Square 0.1 0.5 Average Seed Length 510 ± 70 µm 460 ± 60 µm Average Embryo Length 110 ± 60 µm 70 ± 30 µm