SeedGenes Profile: EMB 2759

Gene Information Gene Symbol EMB 2759 Chromosome Locus At5g63050   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details None Gene Evidence Double Border Recovery Meinke / Syngenta (3 alleles) Single Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2004 Predicted Sequences Gene: 2561 nucleotides mRNA: 1496 nucleotides Protein:   345 amino acids cDNA Status FLcDNA Available: BT011233 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (78%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 247414_at

Mutant Information Allele Symbol emb 2759-1 emb 2759-2 emb 2759-3 emb 2759-4 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Mutant Line Number 11833 16089 123563 123345 Ecotype Columbia Columbia Columbia Columbia Genetic Background         ABRC Stock         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Details Details Location of Mutation Exon 3 Intron 3 to Exon 4 Intron 2 Intron 3 Terminal Phenotype Transition  Details Globular  Details Transition  Details Transition  Details Nomarski Phenotype   Images   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release September, 2004 September, 2004 September, 2004 September, 2004 Seed Color White White White to Pale Yellow White Embryo Color White White White to Pale Yellow White Percent Mutant Seeds 25.6 % 24.8 % 23.7 % 28.2 % Chi - Square 0.2 0.2 1.0 5.5 Seeds Counted 793 888 1121 988 Percent Top Half 48.8 % 52.3 % 58.3 % 48.7 % Chi - Square 0.1 0.5 7.3 0.2 Average Seed Length 510 ± 70 µm 460 ± 60 µm 520 ± 60 µm 540 ± 60 µm Average Embryo Length 110 ± 60 µm 70 ± 30 µm 80 ± 40 µm 100 ± 50 µm