SeedGenes Profile: SUS 1

Gene Information Gene Symbol SUS 1 Chromosome Locus At1g01040   Alleles in Database 7   See List OR Show All Identity Confidence Confirmed Alias Symbol SIN 1 (A. Ray) Sequence Identity CAF (S. Jacobsen) Sequence Identity DCL 1 (A. Ray) Alternate Name EMB 76 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function RNA Helicase Function Details Similar in sequence to a Drosophila protein (Dicer) involved in cleavage of dsRNAs and gene silencing during development Gene Evidence Sequenced Mutant Allele A. Ray (1 allele) Double Border Recovery Meinke / Syngenta (4 alleles) Database Release March, 2002 Predicted Sequences Gene: 8082 nucleotides mRNA: 6251 nucleotides Protein:   1909 amino acids cDNA Status FLcDNA Available: AF292940 Top BLAST Hits Arabidopsis: AT3G43920.1 (37%) Plant ESTs: Medicago truncatula (70%) Model Genomes:   Caenorhabditis elegans (31%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261584_at

Mutant Information Allele Symbol sus 1-4 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 9156 Ecotype Columbia Genetic Background   ABRC Stock CS16068 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 15 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White to Pale Yellow Embryo Color White to Pale Yellow Percent Mutant Seeds 24.5 % Chi - Square 0.1 Seeds Counted 1064 Percent Top Half 54.0 % Chi - Square 1.7 Average Seed Length 470 ± 40 µm Average Embryo Length 50 ± 10 µm