SeedGenes Profile: EMB 1379

Gene Information Gene Symbol EMB 1379 Chromosome Locus At5g21140   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Weak similarity to DNA repair protein (Nse1) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2524 nucleotides mRNA: 1357 nucleotides Protein:   312 amino acids cDNA Status FLcDNA Available: BX831078 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (82%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA

Mutant Information Allele Symbol emb 1379-1 emb 1379-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 17358 SALK_136483 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16151 CS24066 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 1 Intron 3 Terminal Phenotype Preglobular  Details Globular  Details Nomarski Phenotype Globular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 26.5 % 26.7 % Chi - Square 1.1 1.3 Seeds Counted 921 846 Percent Top Half 47.5 % 56.2 % Chi - Square 0.6 3.4 Average Seed Length 460 ± 50 µm 470 ± 60 µm Average Embryo Length 15 ± 10 µm 20 ± 10 µm