SeedGenes Profile: EMB 1507

Gene Information Gene Symbol EMB 1507 Chromosome Locus At1g20960   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Spliceosome-Associated RNA Helicase Function Details Similar to human U5 200 kD splicing factor Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Border Recovery Meinke / Syngenta (2 alleles) Database Release September, 2002 Predicted Sequences Gene: 8167 nucleotides mRNA: 6832 nucleotides Protein:   2171 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT2G42270.1 (82%) Plant ESTs: Vitis vinifera (79%) Model Genomes:   Danio rerio (56%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 262800_at

Mutant Information Allele Symbol emb 1507-1 emb 1507-2 emb 1507-4 emb 1507-5 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Mutant Line Number 26145 84939 55843 131837 Ecotype Columbia Columbia Columbia Columbia Genetic Background         ABRC Stock CS16090 CS16091 CS16092 CS16139 Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details     Location of Mutation Exon 1 Exon 1     Terminal Phenotype Globular  Details Globular  Details Globular  Details Globular  Details Nomarski Phenotype   Images   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release September, 2002 September, 2002 September, 2002 September, 2002 Seed Color Pale Yellow Pale Yellow White White Embryo Color Pale Yellow Pale Yellow White White Percent Mutant Seeds 22.9 % 23.2 % 23.8 % 22.8 % Chi - Square 2.9 2.0 0.7 2.8 Seeds Counted 1219 1154 896 1125 Percent Top Half 49.5 % 50.4 % 54.5 % 57.5 % Chi - Square <0.1 <0.1 1.7 4.8 Average Seed Length 500 ± 30 µm 480 ± 30 µm 490 ± 40 µm 490 ± 40 µm Average Embryo Length 90 ± 30 µm 110 ± 50 µm 60 ± 20 µm 110 ± 30 µm