OSU Syngenta vbi

SeedGenes Profile: EMB 2597

Gene Information
Gene Symbol EMB 2597
Chromosome Locus At4g13750   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol NOV (K. Okada) Sequence Identity
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details Plant-specific nuclear protein
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release September, 2003
Predicted Sequences
Gene:12430 nucleotides
mRNA: 8252 nucleotides
Protein:  2729 amino acids
cDNA Status EST Identified: BX828204
Top BLAST Hits
Arabidopsis: AT1G08300.1 (65%)
Plant ESTs: Soybean (Glycine max) (46%)
Model Genomes:  Danio rerio (28%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 254731_at
    
Mutant Information
Allele Symbol emb 2597-1emb 2597-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Salk
Mutant Line Number 124146SALK_096246
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16217CS24087
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Upstream to Exon 1Exon 1
Terminal Phenotype Cotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release September, 2003March, 2004
Seed Color White to Pale YellowWhite
Embryo Color White to Pale YellowWhite
Percent Mutant Seeds 23.0 %25.7 %
Chi - Square 2.20.4
Seeds Counted 10311197
Percent Top Half 52.3 %55.5 %
Chi - Square 0.53.8
Average Seed Length 410 ± 80 µm420 ± 70 µm
Average Embryo Length 110 ± 110 µm120 ± 120 µm