SeedGenes Profile: PDE 277

Gene Information Gene Symbol PDE 277 Chromosome Locus At2g26930   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol ISPE (TAIR) Alternate Name Gene Class Seed Pigment Predicted Function Isoprenoid Biosynthesis in Plastids Function Details Enzyme in deoxyxylulose phosphate pathway of terpenoid biosynthesis Gene Evidence Border Recovery Chen / Salk (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2002 Predicted Sequences Gene: 2560 nucleotides mRNA: 1311 nucleotides Protein:   383 amino acids cDNA Status FLcDNA Available: AY136394 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (89%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 266863_at

Mutant Information Allele Symbol pde 277 ispE-1 Mutant Class Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Chen / Salk Mutant Line Number 38108 SALK_107310 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16108   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 9 Exon 2 Terminal Phenotype Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Database Release September, 2002 December, 2010 Seed Color White   Embryo Color White   Percent Mutant Seeds 18.9 %   Chi - Square 21.6   Seeds Counted 1103   Percent Top Half 62.2 %   Chi - Square 12.4   Average Seed Length     Average Embryo Length