SeedGenes Profile: PDE 316

Gene Information Gene Symbol PDE 316 Chromosome Locus At1g63680   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol APG 13 (K. Shinozaki) Sequence Identity AtMurE (H. Takano) Sequence Identity Gene Class Seed Pigment Predicted Function Similar to Bacterial mur E Protein Function Details Diverged from bacterial and moss proteins; Putative role in transcription Gene Evidence Border Recovery Takano / Salk (1 allele) Border Recovery Takano / Riken (1 allele) Border Recovery Takano / Wisconsin (2 alleles) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2665 nucleotides mRNA: 2328 nucleotides Protein:   767 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (61%) Model Genomes:   Synechocystis (34%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 260292_at

Mutant Information Allele Symbol pde 316 atmurE-1 atmurE-2 atmurE-3 atmurE-4 Mutant Class Seed Pigment Seed Pigment Seed Pigment Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Takano / Salk Takano / Riken Takano / Wisconsin Takano / Wisconsin Mutant Line Number 13763 SALK_126518 RATM13-6090-1 WiscDsLox477-48 WiscDsLox311G11 Ecotype Columbia Columbia Nossen Columbia Columbia Genetic Background           ABRC Stock CS16226         Mutagen Treatment T-DNA T-DNA Transposon T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)         Location of Mutation Exon 1 to Intron 2 Exon 1 Exon 1 Exon 1 Exon 1 Terminal Phenotype Pigment Defective Pigment Defective Pigment Defective Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Sequence Identity Sequence Identity Sequence Identity Database Release March, 2003 December, 2010 December, 2010 December, 2010 December, 2010 Seed Color White         Embryo Color White         Percent Mutant Seeds 25.7 %         Chi - Square 0.3         Seeds Counted 1143         Percent Top Half 46.9 %         Chi - Square 1.1         Average Seed Length           Average Embryo Length