SeedGenes Profile: PDE 319

Gene Information Gene Symbol PDE 319 Chromosome Locus At2g24120   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol SCA 3 (J. Micol) Sequence Identity Gene Class Seed Pigment Predicted Function Chloroplast DNA-Dependent RNA Polymerase Function Details Transcription of chloroplast genes Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Micol / Salk (2 alleles) Database Release September, 2003 Predicted Sequences Gene: 5727 nucleotides mRNA: 3536 nucleotides Protein:   993 amino acids cDNA Status FLcDNA Available: AK229652 Top BLAST Hits Arabidopsis: AT1G68990.1 (59%) Plant ESTs: Nicotiana tabacum (71%) Model Genomes:   Saccharomyces cerevisiae (38%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 265991_at

Mutant Information Allele Symbol pde 319 sca 3-2 sca 3-3 Mutant Class Seed Pigment Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Micol / Salk Micol / Salk Mutant Line Number 11913 SALK_093884 SALK_067191 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock CS16229     Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Exon 2 Intron 3 Exon 18 Terminal Phenotype Pigment Defective Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Sequence Identity Database Release September, 2003 October, 2007 October, 2007 Seed Color Pale Yellow     Embryo Color Pale Yellow     Percent Mutant Seeds 25.5 %     Chi - Square 0.1     Seeds Counted 1143     Percent Top Half 50.5 %     Chi - Square <0.1     Average Seed Length       Average Embryo Length