SeedGenes Profile: EMB 1275

Gene Information Gene Symbol EMB 1275 Chromosome Locus At1g55350   Alleles in Database 6   See List OR Show All Identity Confidence Confirmed Alias Symbol AtDEK 1 (Olsen; Ingram) Sequence Identity EMB 80 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Calpain-Type Cysteine Protease Function Details Similar to maize DEK1 Gene Evidence Border Recovery Olsen / Dellaporta (1 allele) Border Recovery Olsen / GABI (1 allele) Border Recovery Ingram / Salk (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2004 Predicted Sequences Gene: 10803 nucleotides mRNA: 7220 nucleotides Protein:   2151 amino acids cDNA Status FLcDNA Available: AY061803 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (70%) Model Genomes:   Danio rerio (34%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 259646_at

Mutant Information Allele Symbol emb 1275-1 emb 1275-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 16696 102227 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Intron 5 to Exon 6 Exon 29 Terminal Phenotype Preglobular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2004 September, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 21.8 % 35.5 % Chi - Square 5.6 54.8 Seeds Counted 1010 926 Percent Top Half 53.6 % 50.2 % Chi - Square 1.2 <0.1 Average Seed Length 460 ± 70 µm 530 ± 70 µm Average Embryo Length 15 ± 0 µm 60 ± 30 µm