SeedGenes Profile: EMB 1507

Gene Information Gene Symbol EMB 1507 Chromosome Locus At1g20960   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Spliceosome-Associated RNA Helicase Function Details Similar to human U5 200 kD splicing factor Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Border Recovery Meinke / Syngenta (2 alleles) Database Release September, 2002 Predicted Sequences Gene: 8167 nucleotides mRNA: 6832 nucleotides Protein:   2171 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT2G42270.1 (82%) Plant ESTs: Vitis vinifera (79%) Model Genomes:   Danio rerio (56%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 262800_at

Mutant Information Allele Symbol emb 1507-1 emb 1507-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 26145 84939 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16090 CS16091 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 1 Exon 1 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 September, 2002 Seed Color Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Percent Mutant Seeds 22.9 % 23.2 % Chi - Square 2.9 2.0 Seeds Counted 1219 1154 Percent Top Half 49.5 % 50.4 % Chi - Square <0.1 <0.1 Average Seed Length 500 ± 30 µm 480 ± 30 µm Average Embryo Length 90 ± 30 µm 110 ± 50 µm