OSU Syngenta vbi

SeedGenes Profile: EMB 1644

Gene Information
Gene Symbol EMB 1644
Chromosome Locus At5g27720   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function U6 snRNA Associated Protein (LSm4)
Function Details Component of spliceosome involved in pre-mRNA splicing
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2003
Predicted Sequences
Gene:2044 nucleotides
mRNA: 912 nucleotides
Protein:  129 amino acids
cDNA Status FLcDNA Available: AY087911
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Medicago truncatula (98%)
Model Genomes:  Drosophila melanogaster (82%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 246741_at
    
Mutant Information
Allele Symbol emb 1644-2emb 1644-1
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 3218750481
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16156CS16157
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help)Details
Location of Mutation Exon 5 to DownstreamExon 1 in 5' UTR
Terminal Phenotype Globular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2003
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 24.0 %24.5 %
Chi - Square 0.60.2
Seeds Counted 11161046
Percent Top Half 50.4 %45.7 %
Chi - Square <0.11.9
Average Seed Length 520 ± 50 µm510 ± 60 µm
Average Embryo Length 30 ± 20 µm40 ± 20 µm