SeedGenes Profile: EMB 2360

Gene Information Gene Symbol EMB 2360 Chromosome Locus At3g54660   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast Glutathione Reductase Function Details Protection against oxidative stress Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2004 Predicted Sequences Gene: 3439 nucleotides mRNA: 2196 nucleotides Protein:   565 amino acids cDNA Status FLcDNA Available: AY054677 Top BLAST Hits Arabidopsis: AT3G24170.1 (52%) Plant ESTs: Cotton (Gossypium) (83%) Model Genomes:   Danio rerio (47%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 251860_at

Mutant Information Allele Symbol emb 2360-1 emb 2360-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 125807 80750 Ecotype Columbia Columbia Genetic Background   qrt ABRC Stock CS24049 CS24050 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Exon 1 Intron 4 Terminal Phenotype Preglobular  Details Globular  Details Nomarski Phenotype Globular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2004 March, 2004 Seed Color Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Percent Mutant Seeds 26.0 % 24.9 % Chi - Square 0.5 <0.1 Seeds Counted 1012 1172 Percent Top Half 51.3 % 48.3 % Chi - Square <0.1 0.3 Average Seed Length 490 ± 60 µm 500 ± 60 µm Average Embryo Length 20 ± 10 µm 20 ± 20 µm