OSU Syngenta vbi

SeedGenes Profile: EMB 2360

Gene Information
Gene Symbol EMB 2360
Chromosome Locus At3g54660   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Chloroplast Glutathione Reductase
Function Details Protection against oxidative stress
Gene Evidence
Single Border Recovery
Meinke / Syngenta (1 allele)
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2004
Predicted Sequences
Gene:3439 nucleotides
mRNA: 2196 nucleotides
Protein:  565 amino acids
cDNA Status FLcDNA Available: AY054677
Top BLAST Hits
Arabidopsis: AT3G24170.1 (52%)
Plant ESTs: Cotton (Gossypium) (83%)
Model Genomes:  Danio rerio (47%)
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 251860_at
    
Mutant Information
Allele Symbol emb 2360-1emb 2360-2emb 2360-3
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SyngentaMeinke / Syngenta
Mutant Line Number 1258078075064947
Ecotype ColumbiaColumbiaColumbia
Genetic Background  qrtqrt
ABRC Stock CS24049CS24050CS24051
Mutagen Treatment T-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)DetailsDetails
Location of Mutation Exon 1Intron 4Intron 5
Terminal Phenotype Preglobular  DetailsGlobular  DetailsPreglobular  Details
Nomarski Phenotype Globular  Images  Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release March, 2004March, 2004March, 2004
Seed Color Pale YellowPale YellowWhite
Embryo Color Pale YellowPale YellowWhite
Percent Mutant Seeds 26.0 %24.9 %34.1 %
Chi - Square 0.5<0.122.2
Seeds Counted 10121172508
Percent Top Half 51.3 %48.3 %56.1 %
Chi - Square <0.10.32.6
Average Seed Length 490 ± 60 µm500 ± 60 µm530 ± 60 µm
Average Embryo Length 20 ± 10 µm20 ± 20 µm30 ± 50 µm