SeedGenes Profile: EMB 2369

Gene Information Gene Symbol EMB 2369 Chromosome Locus At4g04350   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Leucyl tRNA Synthetase Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Genoplante (1 allele) Database Release March, 2004 Predicted Sequences Gene: 5040 nucleotides mRNA: 3060 nucleotides Protein:   973 amino acids cDNA Status FLcDNA Available: AY079159 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (79%) Model Genomes:   Synechocystis (45%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 255328_at

Mutant Information Allele Symbol emb 2369-1 emb 2369-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Genoplante Mutant Line Number 81281 EVM17 Ecotype Columbia Wassilewskija Genetic Background qrt   ABRC Stock CS24052 CS24099 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 9 to Exon 10 Exon 6 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2004 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.8 % 25.8 % Chi - Square <0.1 0.4 Seeds Counted 1064 1159 Percent Top Half 45.8 % 52.2 % Chi - Square 1.8 0.6 Average Seed Length 530 ± 70 µm 430 ± 60 µm Average Embryo Length 80 ± 30 µm 70 ± 40 µm