SeedGenes Profile: EMB 2411

Gene Information Gene Symbol EMB 2411 Chromosome Locus At1g08840   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Helicase/Nuclease (Dna2) Function Details DNA replication; Okazaki fragment metabolism Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 8791 nucleotides mRNA: 3891 nucleotides Protein:   1296 amino acids cDNA Status Unknown Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (54%) Model Genomes:   Neurospora crassa (34%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 264801_at

Mutant Information Allele Symbol emb 2411-1 emb 2411-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Genoplante Mutant Line Number 110058 DYE165 Ecotype Columbia Wassilewskija Genetic Background     ABRC Stock CS24053 CS24093 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 10 to Intron 10 Exon 20 Terminal Phenotype Globular  Details Preglobular  Details Nomarski Phenotype   Images Globular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2004 March, 2004 Seed Color Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Percent Mutant Seeds 27.1 % 28.3 % Chi - Square 2.1 5.1 Seeds Counted 863 876 Percent Top Half 50.4 % 58.5 % Chi - Square <0.1 7.1 Average Seed Length 530 ± 60 µm 490 ± 70 µm Average Embryo Length 30 ± 20 µm 20 ± 0 µm