SeedGenes Profile: EMB 2597

Gene Information Gene Symbol EMB 2597 Chromosome Locus At4g13750   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol NOV (K. Okada) Sequence Identity Gene Class Embryo Defective Predicted Function Uncertain Function Details Plant-specific nuclear protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release September, 2003 Predicted Sequences Gene: 12430 nucleotides mRNA: 8252 nucleotides Protein:   2729 amino acids cDNA Status EST Identified: BX828204 Top BLAST Hits Arabidopsis: AT1G08300.1 (65%) Plant ESTs: Soybean (Glycine max) (46%) Model Genomes:   Danio rerio (28%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 254731_at

Mutant Information Allele Symbol emb 2597-1 emb 2597-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 124146 SALK_096246 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16217 CS24087 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Upstream to Exon 1 Exon 1 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2003 March, 2004 Seed Color White to Pale Yellow White Embryo Color White to Pale Yellow White Percent Mutant Seeds 23.0 % 25.7 % Chi - Square 2.2 0.4 Seeds Counted 1031 1197 Percent Top Half 52.3 % 55.5 % Chi - Square 0.5 3.8 Average Seed Length 410 ± 80 µm 420 ± 70 µm Average Embryo Length 110 ± 110 µm 120 ± 120 µm