SeedGenes Profile: EMB 2167

Gene Information Gene Symbol EMB 2167 Chromosome Locus At3g52590   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol HAP 4 (M. Johnson) Alternate Name Gene Class Embryo Defective Predicted Function Ubiquitin Fused to Ribosomal Protein L40 (UbA52) Function Details Ubiquitin ribosomal fusion protein Gene Evidence Single Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2003 Predicted Sequences Gene: 1304 nucleotides mRNA: 677 nucleotides Protein:   128 amino acids cDNA Status FLcDNA Available: AY085820 Top BLAST Hits Arabidopsis: AT2G36170.1 (100%) Plant ESTs: Spruce (99%) Model Genomes:   Homo sapiens (92%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 252056_at

Mutant Information Allele Symbol emb 2167-1 emb 2167-3 emb 2167-4 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Genoplante Mutant Line Number 68089 61713 EYJ5 Ecotype Columbia Columbia Wassilewskija Genetic Background qrt qrt   ABRC Stock CS16168 CS16210 CS24095 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details   Location of Mutation 5' UTR Intron 4 to Exon 5 Intron 3 Terminal Phenotype Transition  Details Globular  Details Globular  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2003 March, 2003 March, 2004 Seed Color White to Pale Yellow White White Embryo Color White to Pale Yellow White White Percent Mutant Seeds 24.4 % 11.3 % 19.9 % Chi - Square 0.1 87.6 15.8 Seeds Counted 435 870 1136 Percent Top Half 50.9 % 58.2 % 56.2 % Chi - Square <0.1 2.6 3.5 Average Seed Length 470 ± 50 µm 430 ± 60 µm 420 ± 60 µm Average Embryo Length 110 ± 50 µm 50 ± 40 µm 50 ± 20 µm