SeedGenes Profile: PDE 129

Gene Information Gene Symbol PDE 129 Chromosome Locus At5g62790   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol DXR (L.J. Qu) Sequence Identity Gene Class Seed Pigment Predicted Function 1-Deoxyxylulose 5-Phosphate Reductoisomerase Function Details Biosynthesis of isoprenoids required for normal chloroplast development Gene Evidence Border Recovery Qu / GABI (1 allele) Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 3315 nucleotides mRNA: 1814 nucleotides Protein:   477 amino acids cDNA Status FLcDNA Available: AY054682 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (94%) Model Genomes:   Synechocystis (65%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 247401_at

Mutant Information Allele Symbol pde 129-1 pde 129-2 dxr Mutant Class Seed Pigment Seed Pigment Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Syngenta Qu / GABI Mutant Line Number 4036 80523 GABI_215C01 Ecotype Columbia Columbia Columbia Genetic Background   qrt   ABRC Stock CS16060 CS16147   Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence   Details  (Help)   Location of Mutation   Intron 3 Exon 7 Terminal Phenotype Pigment Defective Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Database Release March, 2002 September, 2002 December, 2010 Seed Color White White   Embryo Color White White   Percent Mutant Seeds 29.3 % 23.6 %   Chi - Square 9.7 1.2   Seeds Counted 986 1201   Percent Top Half 52.9 % 45.8 %   Chi - Square 1.0 2.0   Average Seed Length       Average Embryo Length