SeedGenes Profile: SUS 2

Gene Information Gene Symbol SUS 2 Chromosome Locus At1g80070   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 177 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function PRP8 Splicing Factor Function Details Pre-mRNA processing (PRP) factor and U5 snRNP component required for spliceosome function Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2002 Predicted Sequences Gene: 9760 nucleotides mRNA: 7386 nucleotides Protein:   2382 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT4G38780.1 (93%) Plant ESTs: Rice (Oryza sativa) (93%) Model Genomes:   Homo sapiens (82%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 262068_at

Mutant Information Allele Symbol sus 2-4 sus 2-5 sus 2-1 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Feldmann Mutant Line Number 69634 69077 6833 Ecotype Columbia Columbia Wassilewskija Genetic Background qrt qrt   ABRC Stock CS16072 CS16073 CS84715 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details   Location of Mutation Exon 22 Exon 2 to Intron 3 Uncertain Terminal Phenotype Globular  Details Globular  Details Transition  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 March, 2002 Seed Color Pale Yellow Pale Yellow Pale Yellow-Green Embryo Color Pale Yellow Pale Yellow Pale Yellow-Green Percent Mutant Seeds 26.4 % 27.3 % 27.8 % Chi - Square 1.0 2.5 4.1 Seeds Counted 1051 852 982 Percent Top Half 48.4 % 51.1 % 46.9 % Chi - Square 0.3 0.1 1.1 Average Seed Length 510 ± 50 µm 540 ± 40 µm 480 ± 40 µm Average Embryo Length 160 ± 40 µm 120 ± 30 µm 110 ± 60 µm